International Journal of Pharmacology and Clinical Sciences, 2013, 2, 1, 14-18.
Published: March 2013
Type: Research Article
Authors: Hardik Soni, Ghanshyam Patel, Megha Shah, Mayank Panchal, and Krishna Murti
Author(s) affiliations:
Hardik Soni1*, Ghanshyam Patel1, Megha Shah2, Mayank Panchal2, Krishna Murti3
1Vasu Research Centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A, GIDC, Makarpura, Vadodara, Gujarat, India.
2Department of Pharmacognosy, Vidyabharti Trust College of Pharmacy, Umrakh, Gujarat, India.
3Department of Pharmacology, Vidyabharti Trust College of Pharmacy, Umrakh, Gujarat, India.
Abstract
Background: Rheumatoid arthritis is a chronic multi-system disease of unknown cause. It affects people in their prime of life, predominantly between the ages of 20-50 years with unpredictable course. Dazzle ointment is a polyherbal formulation, used for the treatment of inflammation and rheumatoid arthritis. Objective: The present study was planned to evaluate efficacy of Dazzle ointment using complete Freund’s adjuvant-induced arthritic model. Materials and Methods: The animals were divided into three groups of 6 animals each as CFA treated, standard drug and test drug treated groups. The animals were injected with 0.2 ml of complete Freund’s adjuvant into the sub-plantar surface of left hind paw. Drugs were administered topically, once a day, commenced on the day of injection of adjuvant and continued for 21 days. The assessment of the change in the inflammatory reaction was made by measuring the paw volume plethysmographically on 0, 4th, 14th, 17th, 21st day after injection of complete Freund’s adjuvant (CFA). The statistical significance was assessed using one-way analysis of variance (ANOVA) followed by Dunnet’s comparison test. A p value of < 0.05 was considered as statistically significant. Results: It was observed that Dazzle ointment produced significant anti-arthritic effect on 21st day. In CFA treated group, there was marked increase in the ESR and WBC count which was significantly decreased by test drug Dazzle ointment and standard drug diclofenac gel (Omni gel). Conclusion: The results indicate that Dazzle ointment possesses anti-arthritic activity in the experimental animal model.