International Journal of Pharmacology and Clinical Sciences, 2013, 2, 3, 67-74.
Published: September 2013
Type: Research Article
Authors: Amoolya Gowda, Venkatesh Shanbhag, Smita Shenoy, and Eesha Rao Bangalore
Amoolya Gowda1, Venkatesh Shanbhag2, Smita Shenoy1, Eesha Rao Bangalore1*, Krishnananda Prabhu3, Raghu Murthy1, Nelluri Venumadhav4, Prashanth kumar Goudapalla3, Mukunda Narayanareddy5, Tara Shanbhag6
1Department of Pharmacology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
2Department of Surgery, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
3Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
4Department of Anatomy, Melaka Manipal Medical College,(Manipal Campus) Manipal University, Manipal, Karnataka, India.
5Department of Pharmacology, Ramaiah Medical College, Bangalore, India.
6Department of Pharmacology, Srinivasa Institute of Medical Sciences,Surathkal
Abstract
Background: The plant Michelia champaca (MC) is widely used in the treatment of inflammation, constipation, dysmenorrhea, ulcers, wounds, fever and cough. The objective of the study was to evaluate the wound healing property of topical application of ethanolic extract of MC flowers in streptozotocin induced diabetes in rats. Materials and Methods: Wound healing activity was assessed by incision and excision wound models. Five groups of 10 rats each were used for each of incision and excision wound model. Group I rats, non-diabetic control and group II rats diabetic control, were anointed topically with ointment base. The diabetic rats in test groups III, IV and V were anointed with topical MC extract ointment of 2.5%, 5% and 10% respectively. Parameters observed were breaking strength of incision wound and wound contraction, epithelialization, hydroxyproline content of excision wound respectively. Results were analyzed by one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test. Results: Breaking strength, rate of wound contraction and hydroxyproline content were significantly increased and period of epithelialization was significantly reduced in Group IV and V rats respectively. Conclusion: The topical application of ethanolic extract of Michelia champaca promotes wound healing in diabetic rats. Hence further study in humans is suggested.